High-throughput biology of cancer and immunity
We use multi-omics profiling and CRISPR technology to discover exciting new biology in cancer and immunity, and we take the most interesting results forward, together with our clinical partners to make them useful for precision medicine.
The biological concept of cellular identity connects a cell’s past with its future potential. Our research is based on the hypothesis that epigenetic cell states and the “epigenetic landscape” constitute a highly informative intermediate layer of information processing – one that captures a cell’s regulatory identity and retains the flexibility to respond swiftly to a broad range of perturbations. This definition of the “epigenetic landscape” is not restricted to specific epigenetic marks such as DNA methylation and histone modifications. Rather, it reflects the full range of transcription regulation by which cells translate various inputs into sustainable changes in their cell state. In particular, the epigenetic landscape not only defines a cell’s current state, but also details its developmental past (e.g., cell-of-origin in cancer) and future trajectories (e.g., plasticity in response to drug treatment).
To study the fundamental role of epigenetic and transcription-regulatory programs in cancer and immunity, we have established high-throughput pipelines to profile gene expression (i.e., bulk and single-cell RNA-seq) and transcriptional regulation (e.g., ATAC-seq, RRBS, ChIPmentation) to dissect the epigenetic landscape in patient cohorts and in disease-relevant mouse models. For example, we have established genome-scale maps of tumor heterogeneity in cancers of children (Ewing sarcoma, Langerhans histiocytosis) and elderly patients (glioblastoma, chronic lymphocytic leukemia). Our work also focuses on immune gene regulation – the first single-cell DNA methylation maps of hematopoietic stem cell differentiation and the first systematic map of immune regulation in non-immune cells (“structural immunity”).
Publications
Multi-omics analysis of innate and adaptive responses to BCG vaccination reveals epigenetic cell states that predict trained immunity
Moorlag SJCFM#, Folkman L#, ter Horst R#, Krausgruber T#, Barreca D, Schuster LC, Fife V, Matzaraki V, Li W, Reichl S, Mourits VP, Koeken VACM, de Bree CJ, Dijkstra H, Lemmers H, van Cranenbroek B, van Rijssen E, Koenen HJP, Jossten I, Xu CJ, Li Y, Joosten LAB, van Crevel R, Netea M#*, Bock C#*
Immunity 57/1, 171-187 (2024). DOI: 10.1016/j.immuni.2023.12.005
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Single-cell and spatial transcriptomics reveal aberrant lymphoid developmental programs driving granuloma formation
Krausgruber T, Redl A#, Barreca D#, Doberer K, Romanovskaia D, Dobnikar L, Guarini M, Unterluggauer L, Kleissl L, Atzmüller D, Mayerhofer C, Kopf A, Saluzzo S, Lim CX, Rexie P, Weichhart T, Bock C*, Stary G*
Immunity 56, 289-306 (2023). DOI: 10.1016/j.immuni.2023.01.014
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Multimodal analysis of cell-free DNA whole-genome sequencing for pediatric cancers with low mutational burden
Peneder P, Stütz AM#, Surdez D, Krumbholz M, Semper S, Chicard M, Sheffield NC, Pierron G, Lapouble E, Totzl M, Erguner B, Barreca D, Rendeiro AF, Agaimy A, Boztug H, Engstler G, Dworzak M, Bernkopf M, Taschner-Mandl S, Ambros IM, Myklebost O, Marec-Bérard P, Burchill SA, Brennan B, Strauss SJ, Whelan J, Schleiermacher G, Schaefer C, Dirksen U, Hutter C, Boye K, Ambros PF, Delattre O, Metzler M, Bock C*, Tomazou EM*
Nature Communications 12, 3230 (2021). DOI: 10.1038/s41467-021-23445-w
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Structural cells are key regulators of organ-specific immune response
Krausgruber T, Fortelny N#, Fife-Gernedl V, Senekowitsch M, Schuster LC, Nemc A, Schmidl C, Rendeiro AF, Lercher A, Bergthaler A, Bock C*
Nature 583, 296-302 (2020). DOI: 10.1038/s41586-020-2424-4
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Chromatin mapping and single-cell immune profiling defines the temporal dynamics of ibrutinib drug response in chronic lymphocytic leukemia
Rendeiro AF, Krausgruber T#, Fortelny N, Zhao F, Penz T, Farlik M, Schuster LC, Kuchler A, Tasnády S, Réti M, Zoltán M, Alpar D#, Bödör C#, Schmidl C#, Bock C*
Nature Communications 11, 577 (2020). DOI: 10.1038/s41467-019-14081-6
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Epigenomics and single-cell sequencing define a developmental hierarchy in Langerhans cell histiocytosis
Halbritter F, Farlik M#, Schwentner R, Jug G, Fortelny N, Schnöller T, Pisa H, Schuster LC, Reinprecht A, Czech T, Gojo J, Holter W, Minkov M, Bauer WM, Simonitsch-Klupp I, Bock C*, Hutter C*
Cancer Discovery 9, 1406-1421 (2019). DOI: 10.1158/2159-8290.CD-19-0138
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Combined chemosensitivity and chromatin profiling prioritizes drug combinations in CLL
Schmidl C, Vladimer GI#, Rendeiro AF#, Schnabl S#, Krausgruber T, Taubert C, Krall N, Pemovska T, Araghi M, Snijder B, Hubmann R, Ringler A, Runggatscher K, Demirtas D, de la Fuente OL, Hilgarth M, Skrabs C, Porpaczy E, Gruber M, Hoermann G, Kubicek S, Staber PB, Shehata M#, Superti-Furga G#, Jäger U#, Bock C*
Nature Chemical Biology 15, 232-240 (2019). DOI: 10.1038/s41589-018-0205-2
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The DNA methylation landscape of glioblastoma disease progression shows extensive heterogeneity in time and space
Klughammer J, Kiesel B#, Roetzer T, Fortelny N, Nemc A, Nenning KH, Furtner J, Sheffield NC, Datlinger P, Peter N, Nowosielski M, Augustin M, Mischkulnig M, Strobel T, Alpar D, Ergüner B, Senekowitsch M, Moser P, Freyschlag CF, Kerschbaumer J, Thomé C, Grams AE, Stockhammer G, Kitzwoegerer M, Oberndorfer S, Marhold F, Weis S, Trenkler J, Buchroithner J, Pichler J, Haybaeck J, Krassnig S, Mahdy Ali K, von Campe G, Payer F, Sherif C, Preiser J, Hauser T, Winkler PA, Kleindienst W, Wurtz F, Brandner-Kokalj T, Stultschnig M, Schweiger S, Dieckmann K, Preusser M, Langs G, Baumann B, Knosp E, Widhalm G, Marosi C, Hainfellner JA, Woehrer A#, Bock C
Nature Medicine 24, 1611-1624 (2018). DOI: 10.1038/s41591-018-0156-x
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DNA methylation heterogeneity defines a disease spectrum in Ewing sarcoma
Sheffield NC, Pierron G, Klughammer J, Datlinger P, Schönegger A, Schuster M, Hadler J, Surdez D, Guil-lemot D, Lapouble E, Freneaux P, Champigneulle J, Bouvier R, Walder D, Ambros IM, Hutter C, Sorz E, Am-aral AT, de Alava E, Schallmoser K, Strunk D, Rinner B, Liegl-Atzwanger B, Huppertz B, Leithner A, de Pin-ieux G, Terrier P, Laurence V, Michon J, Ladenstein R, Holter W, Windhager R, Dirksen U, Ambros PF, Delat-tre O, Kovar H, Bock C*, Tomazou EM*
Nature Medicine 23, 386-395 (2017). DOI: 10.1038/nm.4273
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Specification of tissue-resident macrophages during organogenesis
Mass E, Ballesteros I#, Farlik M#, Halbritter F#, Günther P, Crozet L, Jacome-Galarza CE, Händler K, Klughammer J, Kobayashi Y, Gomez-Perdiguero E, Schultze JL, Beyer M#, Bock C#, Geissmann F*
Science 353, 6304 (2016). DOI: 10.1126/science.aaf4238
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Chromatin accessibility maps of chronic lymphocytic leukaemia identify subtype-specific epigenome signatures and transcription regulatory networks
Rendeiro AF, Schmidl C#, Strefford JC#, Walewska R, Davis Z, Farlik M, Oscier D, Bock C*
Nature Communications 7, 11938 (2016). DOI: 10.1038/ncomms11938
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* shared first or shared senior authorship