ChIPmentation

Low-input and high-throughput chromatin mapping

ChIPmentation

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.

Publication

ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors

ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors

Schmidl C*, Rendeiro AF*, Sheffield NC, Bock C

Nature Methods  12, 963-965 (2015). DOI: 10.1038/nmeth.3542

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* shared first or shared senior authorship